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Mutation of H143 to an asparagine resulted while in the remarkable lack of catalytic action and a average boost in the K
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The effects of solvent viscosity on the catalytic level constants link naga26 with the hydrolysis of one by Zn-NagA are revealed in Figures 4A and 4B using sucrose because the micro-viscogen. The plot of relative k
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m vs. pH-profile for Zn-NagA is revealed in Figure 2B. With this profile, catalytic exercise is shed at substantial and very low values of pH and the data had been in shape to equation four where by two teams, in both the substrate or totally free enzyme, with equivalent p